Center for Medical Research

Medizinische Universität Graz

Basics of mass spectrometry and chromatography

Course Content

In this class you will learn about all the basic principles of chromatography and mass spectrometry and some selected application examples will be shown. The basics of chromatography will include mechanisms of separations and various stationary phases like reversed phase, normal phase, size exclusion chromatography etc. Furthermore, different chromatographic techniques like high performance liquid chromatography (HPLC), gas chromatography (GC) or solid phase extraction (SPE) will be on the agenda. You will also learn which samples demand which chromatographic separation type. The second technology covered will be mass spectrometry. On this side you will learn how a mass spectrometer works and which principal components it contains. Various sorts of instrumentation like quadrupole, triple quadrupole, Orbitrap, MALDI-TOF etc. will be topics of this class. Another important point will be coupling of mass spectrometry technology to chromatography. Finally applications of this hyphenated instrumentation will be shown. Different sample preparation techniques in lipidomics and metabolomics like liquid-liquid extractions will also be part of this class. The applications will be centered on two key applicative models of chromatography-mass spectrometry: targeted methods and non-targeted methods applied to topics in lipidomics and metabolomics. In this respect bioinformatic processing of lipidomics and metabolomics data will also be covered in this class.

Duration
4 Hours

Participation Fee
150 EUR
(MUG employees, BioTechMed members and academic institutions: Ask for a discount!)

Registration
zmf-sekretariat(at)medunigraz.at

 

 

LC-MS practical

Course Content

This will be a practical, hands-on course that takes place in the labs of the core facility mass spectrometry. The prerequisite for this course is the basics of mass spectrometry and chromatography class. At the beginning of this full day, we will start with sample preparation for lipidomic analysis of a tissue or serum sample. Sample preparation will include extraction of lipids by sample homogenization and tissue disruption (if necessary) and subsequent liquid – liquid extraction by a methyl tert-butyl ether protocol. Following that we will get a short introduction on a triple quadrupole tandem mass spectrometer (MS/MS) coupled to an ultra-high performance liquid chromatography (UHPLC) system. You will see how to calibrate the instrument, how to set up LC-MS methods and finally how to set up and start an acquisition sequence. While the LC-MS data of your previously prepared samples will be acquired by the instrument either in precursor ion scan, neutral loss scan or multiple reaction monitoring (MRM) mode, you will get an overview of our high resolution Orbitrap instrumentation. After data acquisition, you will have to do the data processing with our data analysis software which allows for qualitative and quantitative analysis of your data. During this process you will also learn which options you have for data quantitation.

Duration
1 Day

Participation Fee
490 EUR
(MUG employees, BioTechMed members and academic institutions:
Ask for a discount!)

Registration
zmf-sekretariat@medunigraz.at

GC-MS practical

Course Content

This will be a practical, hands-on course that takes place in the labs of the core facility mass spectrometry. The prerequisite for this course is the basics of mass spectrometry and chromatography class. At the beginning of this full day we will start with sample preparation for fatty acid analysis of a tissue or serum sample. Sample preparation will include extraction of lipids by sample homogenization and tissue disruption (if necessary) and subsequent liquid – liquid extraction by a methyl tert-butyl ether protocol. Following that, the samples will be derivatized by a transmethylation protocol resulting in fatty acid methyl esters, which are more amenable in GC-MS. Thereafter we will get a short introduction on a quadrupole based GC-MS system. You will see how to check the instruments readiness, how to set up GC-MS methods and finally how to set up and start an acquisition sequence. GC-MS data of your previously prepared samples will be acquired by the instrument in electron impact (EI) ionization mode. After data acquisition you will have to do the data processing with our data analysis software which allows for quantitative analysis of your data. During this process you will also learn which options you have for data quantitation. We will also show you how to use the national institute for standards and technology (NIST) database for easy identification of your fatty acids.

Duration
1 Day

Participation Fee
325 EUR
(MUG employees, BioTechMed members and academic institutions:
Ask for a discount!)

Registration
zmf-sekretariat(at)medunigraz.at

 

 

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