A variety of cell-based assays are available to characterize physiological reactions (e.g. viability, apoptosis, necrosis, organelle function, proliferation of cells). The course introduces into selection of the right assay, correct performance, troubleshooting and appropriate statistical evaluation of the results. Practical work includes cell culturing, treatment, analysis by plate reader and practical computer based exercises for correct data analysis. The hands-on workshop contains:
experience in cell culture
350 Euro (University)/ 500 Euro (Company)
Introduction to cytotoxicity screening with an explanation of the most common screening assays (formazan bioreduction, neutral red, ATP-content, etc.) and specific assays for membrane integrity, mitochondrial damage, and oxidative stress. Troubleshooting.
Practical part: exposure of cells to a toxic compound with morphological evaluation, MTS assay, quantification of ATP-content, LDH-release, and combined apoptosis/necrosis detection.
Knowledge of cell culture
(MUG employees, BioTechMed members and Academic Institutions:
Ask for a discount!)
Principles of immunohistochemical staining and organelle staining (stains for life/fixed cells dyes/antibodies), dye staining principles (enzymatic activity/physical interactions), antigen retrieval, controls, choice of detection technique, multicolour staining.
Practical part: staining of tissue sections and cells in different formats: cell culture wells, (chamber) slides, and membranes. Antigen detection with and without antigen retrieval, life organelle staining, colorimetric and fluorescent detection, triple staining.
(MUG employees, BioTechMed members and academic institutions: Ask for a discount!)