CF Flow Cytometrie - Vertrigo Images/adobestock.com - generiert mit KI

We offer a variety of services in high-end analytical flow cytometry, cell sorting, functional assays and immunoassays on a fee-for-service basis. Samples can be dropped off at the core facility to be run by our experienced staff. Alternatively, internal users also have the possiblity to use our equipment independently at no charge after completion of a personalized hands-on training session at the instrument. Cell sorting is typically performed by the facility staff members but the FACSMelody sorter is open to trained users for autonomous use upon request.

Our equipment is available 24/7 for unassisted use whereas staff assisted use is available only during regular business hours. Additional service free of charge includes user consultation, training and continuous support for independent users of our instruments. We are always open to the development and implementation of new methods upon user request. We also have the possibility to subsidize part of the expenses.

A price list showing internal and external rates for our service can be found here.

Our services

Multicolor analytical flow cytometry

  • Phenotypic assays (immunophenotyping, immune cell differentiation/polarization, immune checkpoints, cellular senescence, stem/progenitor  cells, cancer cells, cell lines)
  • Functional assays (stimulation/activation, antigen-specific T cells, T cell degranulation , cytokine secretion)
  • Rare cell detection
  • Quantitation of surface marker expression (antigen density)
  • DNA analysis (DNA ploidy/index, cell cycle)
  • Apoptosis
  • Cell proliferation  and tracking
  • Extracellular vesicles

High-speed cell sorting

  • Multiple bulk sorting (4-way sorting in tubes) with high purity
  • Single cell sorting (on slides or in plates for clonal expansion or single-cell biology research)

Immunological service

  • ELISA („enzyme-linked immunosorbent assay“)
  • Multiplexing (Luminex®) – main application: quantitation of cytokines in cell culture supernatants, serum or plasma
  • Preparation and cryopreservation of PMBCs from human blood

Others

  • Detection and quantitation of nanoparticles (NTA technology; NanoSight NS300)

Training and support

  • Panel design
  • Sample preparation
  • Troubleshooting
  • Data analysis
  • Reviewing of grant proposals and manuscripts (methods, results)
  • Instrument training for independent users
  • Technical assistance
  • Flow cytometry basic course (biannual)

Acknowledging the Core Facility

Please remember to acknowledge the use of our core facility equipment and services in your publications.