Projects

The Core Facility Flow Cytometry supports numerous research projects with specialized analyses and cell sorting. In collaboration with various clinical and preclinical institutions at the Medical University of Graz, complex issues in the fields of immunophenotyping, multiplex assays, and extracellular vesicles are addressed.

Project overview

Sorts

Chimerism analysis – Division of Haematology (Project lead: Annkristin Heine)
Sort of leukocytes from blood or bone marrow of leukemia patients after allogeneic stem cell transplantation with subsequent chimerism analysis (STR)
TP53 mutated AML subclones exhibit engraftment in a humanized bone marrow ossicle mouse model – Division of Haematology (Project lead: Andreas Reinisch)
Sort of human leukocytes from mice bone marrow and ossicle
Pulmonary hypertension – Ludwig Boltzmann Institute for Lung Vascular Research (Project lead: Grazyna Kwapiszewska-Marsh)
Sort of leukocytes from lungs of patients with pulmonary hypertension
Lysophosphatidic acid (LPA)-induced inflammation in microglia – Division of Molecular Biology and Biochemistry, Gottfried Schatz Research Center (Project lead: Wolfgang Sattler)
Sort of CD11b+CD45low microglia from mice brain
Isolation of archea from human gastrointestinal tract (GIT) – D & R Institute of Hygiene, Microbiology and Environmental Medicine (Project lead: Christine Moissl-Eichinger)
Sort of Methanobrevibacter strains from fecal samples

Immunophenotyping

HHV-6 Specific T-Cell Immunity in Healthy Children and Adolescents – Division of Paediatric Haematology-Oncology, Department of Pediatrics and Adolescent Medicine (Project lead: Volker Strenger)
Flow cytometric analysis of immune cells in Human herpesvirus-6
Immune cells in Diarrhea-predominant irritable bowel syndrome (IBS-D) – Division of Gastroenterology and Hepatology (Project lead: Adrian Mathias Moser)
Flow cytometric analysis of immune cells in human mucosal biopsies
Prion Protein in Age-related Macular Degeneration – Department of Ophthalmology (Project lead: Ewald Lindner)
Flow cytometric analysis of prion protein on human leukocytes

Multiplex Assays

Plasma Cytokine Levels in Patients with Invasive Candidiasis – Division of Infectious Diseases, Department of Internal Medicine (Project lead: Robert Krause)
Multiplex immunoassay with 11 analytes
Immune Monitoring Panel 65-plex – Department of Dermatology and Venereology (Project lead: Peter Wolf)
Human Cytokine Screening Panel 48-plex – Evomedis (Project lead: Martin Funk)

Extracellular vesicles

Endothelial microparticles (EMP) in the blood of systemic scleroderma patients – Division for Angiology (Project lead: Philipp Jud)
Flow cytometric analysis of CD31 positive endothelial microparticles in plasma from whole blood
Extracellular vesicles in tumour cell lines – Department of Biomedical Research (Project lead: Beate Rinner)
Flow cytometric analysis of CD9, CD63, CD81 positive extracellular vesicles in cell culture supernatant