ZMF

The Core Facility Molecular Biology (CFMB)offers trendsetting technologies in the field of genome and transcriptome analysis with the focus on Next Generation Sequencing (NGS) based applications. High-quality sample preparation with partially/fully automated pipetting systems ensure standardized workflows for reproducible data.

Labor

Service

  • Automated nucleic acid extractions
  • Automated library preparations for bulk RNA-Sequencing
  • 16S/18S/ITS based microbiome analysis
  • Shot gun Metagenomics and Shallow Sequencing
  • 10X Genomics based single cell analysis (transcriptomics, immunprofiling, cell surface feature barcoding, ATAC)
  • VISIUM Spatial Transcriptomics (protocol for fresh frozen and FFPE material)
  • NGS based CRISPR screening
  • Quantitative Real-time PCR
  • Digital Droplet PCR
  • Nanostring analysis (mRNA, miRNA, PlexSet)

Infrastructure

  • QiaSymphony (Qiagen)
  • Biomek i7 (Beckman)
  • MiSeq (Illumina)
  • Chromium Controller (10X Genomics)
  • Digital Droplet Generator (BioRad)
  • nCounter Prepstation (Nanostring)
  • LC480 Real-time PCR system for 96 and 384 well format (Roche)
  • CFX Touch Real-time PCR system for 96 and 384 well format (BioRad)

Contact

Birgit Gallé 
T: +43 316 385 72828

Nina Schweintzger 
T: +43 316 385 30899
T: +43 316 385 72830